✦ LIBER ✦
Chromatography of human prothrombin from Nitschmann fraction III on DEAE Sepharose Fast Flow using axial and radial flow column
✍ Scribed by Tao Sun; Ge Chen; Yiping Liu; Fengrong Bu; Meijuan Wen
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 68 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0269-3879
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✦ Synopsis
An axial column (3 Â 2.6 cm) and a radial flow column (3.5 Â 5 cm) packed with DEAE Sepharose Fast Flow media was evaluated for the separation of human prothrombin from Nitschmann fraction III. Under radial flow conditions, a sample flow rate up to 14 mL/min (approximately 18 bed vols/h) was achieved. Breakthrough capacity was determined and both columns had almost the same breakthrough capacity per mL media, indicating that the sample loading was independent of radial column geometry.