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Isolation of human fibrinogen by axial and radial flow chromatography from Nitschmann fraction I

✍ Scribed by Sun Tao; Chen Ge; Liu Yipin; Bu Fengrong; Wen Meijuan


Book ID
114420805
Publisher
Springer-Verlag
Year
1999
Tongue
English
Weight
59 KB
Volume
13
Category
Article
ISSN
0951-208X

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Chromatography of human prothrombin from
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An axial column (3 Γ‚ 2.6 cm) and a radial flow column (3.5 Γ‚ 5 cm) packed with DEAE Sepharose Fast Flow media was evaluated for the separation of human prothrombin from Nitschmann fraction III. Under radial flow conditions, a sample flow rate up to 14 mL/min (approximately 18 bed vols/h) was achieve