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Cloning of replication origins from the E. coli K12 chromosome

โœ Scribed by DIAZ, R.; PRITCHARD, R. H.

Book ID
109710948
Publisher
Nature Publishing Group
Year
1978
Tongue
English
Weight
544 KB
Volume
275
Category
Article
ISSN
0028-0836

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๐Ÿ“œ SIMILAR VOLUMES

Location and characterisation of a new r
Location and characterisation of a new replication origin in the E. coli K12 chromosome
โœ Diaz, R. ;Barnsley, P. ;Pritchard, R. H. ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Springer ๐ŸŒ English โš– 639 KB

A segment of DNA located in the region of the E. coli K12 chromosome previously identified by the Rac phenotype can function as a self-replicating plasmid. Evidence is presented that this plasmid, the oriJ plasmid, contains the origin of replication of a defective prophage postulated to be located i

Map position of the replication origin o
Map position of the replication origin on the E. coli chromosome
โœ Fayet, Olivier ;Louarn, Jean-Michel ๐Ÿ“‚ Article ๐Ÿ“… 1978 ๐Ÿ› Springer ๐ŸŒ English โš– 264 KB

Strains carrying a dnaA temperature sensitive (t.s.) mutation and a Mu-1 prophage inserted within different genes near the origin of replication have been constructed. For each strain, integratively suppressed Hfrs, named G and D in which the ori region was replicated clockwise and counterclockwise

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Mini-chromosomes: Plasmids which carry the E. coli replication origin
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We have isolated plasmids by linking the 5.9 MD EcoRI fragment of E. coli that carries the origin of replication to an EcoRI fragment that carries an amplicillin resistance determinant, but lacks an origin of replication. 3 plasmids of this type, pOC1, pOC2, and pOC3, are described in detail in this