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Location and characterisation of a new replication origin in the E. coli K12 chromosome

✍ Scribed by Diaz, R. ;Barnsley, P. ;Pritchard, R. H.

Publisher: Springer
Year: 1979
Tongue: English
Weight: 639 KB
Volume: 175
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00425531

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✦ Synopsis

A segment of DNA located in the region of the E. coli K12 chromosome previously identified by the Rac phenotype can function as a self-replicating plasmid. Evidence is presented that this plasmid, the oriJ plasmid, contains the origin of replication of a defective prophage postulated to be located in this chromosomal region by Low (1973). The plasmid can only be maintained in strains in which this postulated prophage has been deleted. In strains which possess the prophage selection for plasmid maintenance permits the isolation of clones containing new deletions which we postulate are the result of prophage excision.

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