✦ LIBER ✦

Mini-chromosomes: Plasmids which carry the E. coli replication origin

✍ Scribed by Messer, W. ;Bergmans, H. E. N. ;Meijer, M. ;Womack, J. E. ;Hansen, F. G. ;von Meyenburg, K.

Publisher: Springer
Year: 1978
Tongue: English
Weight: 614 KB
Volume: 162
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00268852

No coin nor oath required. For personal study only.

✦ Synopsis

We have isolated plasmids by linking the 5.9 MD EcoRI fragment of E. coli that carries the origin of replication to an EcoRI fragment that carries an amplicillin resistance determinant, but lacks an origin of replication. 3 plasmids of this type, pOC1, pOC2, and pOC3, are described in detail in this report. Although the plasmids have some adverse effect on the growth properties of the host strain, their existence shows that two functioning chromosomal origins can coexist in one cell. Deletions generated from this type of plasmids allow an allocation of the origin of replication of E. coli within a DNA segment less than 0.4 MD in size.

📜 SIMILAR VOLUMES

Map position of the replication origin o

Map position of the replication origin on the E. coli chromosome

✍ Fayet, Olivier ;Louarn, Jean-Michel 📂 Article 📅 1978 🏛 Springer 🌐 English ⚖ 264 KB

Strains carrying a dnaA temperature sensitive (t.s.) mutation and a Mu-1 prophage inserted within different genes near the origin of replication have been constructed. For each strain, integratively suppressed Hfrs, named G and D in which the ori region was replicated clockwise and counterclockwise

Location and characterisation of a new r

Location and characterisation of a new replication origin in the E. coli K12 chromosome

✍ Diaz, R. ;Barnsley, P. ;Pritchard, R. H. 📂 Article 📅 1979 🏛 Springer 🌐 English ⚖ 639 KB

A segment of DNA located in the region of the E. coli K12 chromosome previously identified by the Rac phenotype can function as a self-replicating plasmid. Evidence is presented that this plasmid, the oriJ plasmid, contains the origin of replication of a defective prophage postulated to be located i

Isolation and characterization of λb221p

Isolation and characterization of λb221poriCasnA, a plaque-forming specialized transducing phage carrying the origin of replication of the Escherichia coli chromosome

✍ Soll, Larry 📂 Article 📅 1980 🏛 Springer 🌐 English ⚖ 864 KB

A specialized transducing phage lambda b221poriCasnA has been isolated carrying oriC the origin of chromosomal replication of Escherichia coli. All phage genes required for lytic growth are retained, thus the phage is capable of lytic growth. The presence of the oriC locus confers upon infecting pha