Enzymatic replication of E. coli chromosomal origin is bidirectional
โ Scribed by Kaguni, Jon M.; Fuller, Robert S.; Kornberg, Arthur
- Book ID
- 109726137
- Publisher
- Nature Publishing Group
- Year
- 1982
- Tongue
- English
- Weight
- 521 KB
- Volume
- 296
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/296623a0
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
We have isolated plasmids by linking the 5.9 MD EcoRI fragment of E. coli that carries the origin of replication to an EcoRI fragment that carries an amplicillin resistance determinant, but lacks an origin of replication. 3 plasmids of this type, pOC1, pOC2, and pOC3, are described in detail in this
Strains carrying a dnaA temperature sensitive (t.s.) mutation and a Mu-1 prophage inserted within different genes near the origin of replication have been constructed. For each strain, integratively suppressed Hfrs, named G and D in which the ori region was replicated clockwise and counterclockwise
F' strains of E. coli have been isolated which are merodiploid for various chromosomal segments between 66 and 78 minutes. Strains diploid for the chromosomal DNA between the genes bgl and mtl grow slowly, have a reduced DNA/mass and an increased cell size. These properties could result if the chrom