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Cloning and expression of rat GFAP cDNA inEscherichia coli

✍ Scribed by V. P. Chekhonin; K. A. Pavlov; A. N. Shkoporov; B. A. Yefimov; O. I. Gurina; T. B. Dmitrieva

Publisher
Springer US
Year
2007
Tongue
English
Weight
351 KB
Volume
143
Category
Article
ISSN
0007-4888

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We have cloned the cDNA for luciferase from lantern poly(A)+ RNA of a Japanese firefly, Luciola cruciara (Genji botaru in Japanese). This cDNA directed the synthesis of enzymatically active luciferase under the control of the lac promoter in Escherichia coli. The amino acid sequence predicted from t