Cloning and Expression of Human Neuron-Specific Enolase cDNA inEscherichia coli
β Scribed by K. A. Pavlov; O. I. Gurina; O. M. Antonova; A. V Semenova; V. P. Chekhonin
- Publisher
- Springer US
- Year
- 2011
- Tongue
- English
- Weight
- 474 KB
- Volume
- 152
- Category
- Article
- ISSN
- 0007-4888
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
We have cloned the cDNA for luciferase from lantern poly(A)+ RNA of a Japanese firefly, Luciola cruciara (Genji botaru in Japanese). This cDNA directed the synthesis of enzymatically active luciferase under the control of the lac promoter in Escherichia coli. The amino acid sequence predicted from t
We have isolated and sequenced a cDNA clone encoding the human gamma enolase. Comparison of our cDNA sequence and the rat gamma enolase sequence revealed 97% homology at the level of amino acid sequence. The two coding regions were 91% homologous on the nucleotide level, whereas the 3' noncoding reg