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cDNA cloning and expression of aTalaromyces emersoniiamylase encoding genetic determinant inEscherichia coli

✍ Scribed by L. Bunni; D. C. Coleman; L. McHale; T. J. Hackett; A. P. McHale

Publisher
Springer Netherlands
Year
1992
Tongue
English
Weight
725 KB
Volume
14
Category
Article
ISSN
0141-5492

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Molecular cloning and functional express
Molecular cloning and functional expression of aTalaromyces emersoniiderived alpha-amylase encoding genetic determinant in a human cell line
✍ L. Bunni; T. J. Hackett; L. McHale; G. Flynn; A. P. McHale πŸ“‚ Article πŸ“… 1993 πŸ› Springer Netherlands 🌐 English βš– 440 KB

A eDNA fragment encoding a Talaromyces emersonii acid stable alpha-amylase has beun cloned into a mammalian cell expression vector system. When human HeLa cells were tnmsformed with this DNA, functional enzyme could be detected in extracellular media and cell lysates derived from stable transfonnant