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Cloning and characterization of a cDNA encoding a sulfur-rich coixin

✍ Scribed by Adilson Leite; José Andrés Yunes; Silvia Regina Turcinelli; Paulo Arruda

Publisher
Springer
Year
1992
Tongue
English
Weight
305 KB
Volume
18
Category
Article
ISSN
0167-4412

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✦ Synopsis

A full-length cDNA clone encoding a sulfur-rich Coix prolamin was isolated using a eDNA library constructed from polysomal mRNA prepared from immature Coix endosperm. The deduced amino acid sequence of the eDNA clone predicted a polypeptide of 194 residues, which shared 64~o homology with the 17 kDa fi-zein. The mature protein contains the familiar composition of the prolamins and an unusually high content of the sulfur-containing amino acids methionine (11.6~o) and cysteine (5.2~o). In vitro transcription followed by in vitro translation of the coding region of the pBCX17.9S clone gave rise to a polypeptide with an apparent molecular weight corresponding to the C4 c~-coixin. Hydropathy analysis showed that C4 e-coixin is slightly more hydrophobic than fi-zein.

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