𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Cloning and characterization of a cDNA encoding a Rab1-like small GTP-binding protein fromPetunia hybrida

✍ Scribed by Colette Jako; Bernard Teyssendier Serve

Publisher
Springer
Year
1996
Tongue
English
Weight
388 KB
Volume
31
Category
Article
ISSN
0167-4412

No coin nor oath required. For personal study only.

✦ Synopsis

The cloning of small GTP-binding proteins from Petunia hybrida was performed using a PCR-based strategy. Degenerate primers were designed from the DTAGQE and FMETSA consensus sequences. Three different cDNAs were amplified. The deduced polypeptide sequences PhPCRGP1 and PhPCRGP2 were homologous to RB11_HUMAN and PhPCRGP3 to RAB1A_HUMAN. Using PhPCRGP3 as a probe, 8 identical clones were selected from a Petunia leaf cDNA library. They all encode the same 22.5 kDa polypeptide, PhRAB1, able to bind GTP in vitro and 72% identical to RAB1A_HUMAN. Hybridizable mRNAs encoding PhRAB1 accumulated preferentially in opened flowers.

πŸ“œ SIMILAR VOLUMES

Isolation, characterization, and chromos
Isolation, characterization, and chromosomal mapping of a novel cDNA clone encoding human selenium binding protein
✍ Peter W.G. Chang; Stephen K.W. Tsui; Choong-chin Liew; Cheuk-yu Lee; Mary M.Y. W πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 English βš– 276 KB πŸ‘ 2 views

We have isolated the full-length human 56 kDa selenium binding protein (hSP56) cDNA clone, which is the human homolog of mouse 56 kDa selenium binding protein. The cDNA is 1,668 bp long and has an open reading frame encoding 472 amino acids. The calculated molecular weight is 52.25 kDa and the estim

Molecular cloning of a cDNA for a small
Molecular cloning of a cDNA for a small GTP binding protein, BRho, from the embryo of Bombyx mori and its characterization after expression and purification
✍ Tomohide Uno; Ai Nakasuji; Wajiro Hara; Yasuo Aizono πŸ“‚ Article πŸ“… 2000 πŸ› John Wiley and Sons 🌐 English βš– 149 KB πŸ‘ 2 views

A cDNA clone encoding a small GTP binding protein (BRho) was isolated from an embryonic cDNA library of Bombyx mori that encoded a polypeptide with 202 amino acids sharing 60-80% similarity with the Rho1 family of GTP binding proteins. The effector site and one of the guanine nucleotide binding site