✦ LIBER ✦

Circular dichroism detection in HPLC

✍ Scribed by Piero Salvadori; Carlo Bertucci; Carlo Rosini

Publisher: John Wiley and Sons
Year: 1991
Tongue: English
Weight: 775 KB
Volume: 3
Category: Article
ISSN: 0899-0042
DOI: 10.1002/chir.530030424

No coin nor oath required. For personal study only.

✦ Synopsis

A circular dichroism-based detection system presents several advantages in the HPLC analysis of chiral compounds because of the selective monitoring of optically active molecules. Its use allows reliable determination of enantiomeric excesses and elution order. To this end, the application of empirical, semiempirical, and nonempirical methods to get stereochemical information from the CD signal is reported. Furthermore, recording the CD spectra on line and evaluation of the dissymetry factor make the CD detection very powerful in characterizing the stereochemistry of chiral eluates.

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