HPLC/circular dichroism detection using a split-type flow cell for polarized photometric detector
✍ Scribed by Atsushi Yamamoto; Akinobu Matsunaga; Kazuichi Hayakawa; Masayuki Nishimura
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 141 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0269-3879
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✦ Synopsis
HPLC/circular dichroism detection using a conventional spectrophotometric detector has been established. A split-type flow cell developed for a polarized photometric detector, which is a non-modulated polarimeter, was inserted into this detector. When two rays of circularly polarized light with opposite rotational senses to each other were incident in both cells by combining a polarizer and a 1/4 wave retarder in front of the cell assembly, the output from this spectrophotometric detector became solely the signal based on circular dichroism. Selective detection of a chiral analyte, in which the ratio of the difference in the absorbance between two rays mentioned above and the absorbance by normal rays is over 1/1000, is feasible using the proposed instrument.