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Fluorescence-detected circular dichroism studies of serum albumins

✍ Scribed by Mark P. Thomas; G. Patonay; I.M. Warner

Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
672 KB
Volume
164
Category
Article
ISSN
0003-2697

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✦ Synopsis

Multidimensional fluorescence-detected circular dichroism (FDCD) is used to investigate the binding of bilirubin to four mammalian serum albumins. It is shown that the complexes formed with the albumins have distinctly different FDCD spectra. The effect of the pH-dependent transition from basic to acidic conditions on the complexes is examined. The binding of warfarin to albumin is also presented to demonstrate the general analytical utility of the multidimensional FDCD measurement for biochemical analysis. o 1987 Academic FVSS, I~C.

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