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Reversible binding of ethacrynic acid to human serum albumin: Difference circular dichroism study

✍ Scribed by Carlo Bertucci; Barbara Nanni; Piero Salvadori

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
155 KB
Volume
11
Category
Article
ISSN
0899-0042

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✦ Synopsis

The reversible binding of ethacrynic acid was characterized by a difference circular dichroism method. A 2/1 stoichiometry was determined for the [drug]/[HSA] (human serum albumin) complex. The reversible binding of ethacrynic acid to HSA determines direct competition with ligands that selectivity bind to site II and to the fatty acid site. Furthermore, indirect competition was shown for ligands for site I (anti-cooperative) and to site III (cooperative).

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