Chromatography on Glass of Controlled Pore Size
โ Scribed by HALLER, WOLFGANG
- Book ID
- 109643946
- Publisher
- Nature Publishing Group
- Year
- 1965
- Tongue
- English
- Weight
- 801 KB
- Volume
- 206
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/206693a0
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๐ SIMILAR VOLUMES
The inclusion of urea has been found to eliminate adsorption of proteinsodium dodecyl sulphate (SDS) complexes to controlled pore glass. Using buffer containing 6 M urea, 0.5 "A, SDS and glass with pore diameter 12.3 nm, it is possible to determine protein molecular weights in the range 3500-12,000.
Severztl controlled-pore glslss (CPG) derivatives were examined as supports for the affinity chromatographic purification of the enzyme P-galactosidase. The competitive inhibitor p-aminophenyl$-D-thiogalactopyranoside was coupled to an azelaic acid and a malonic acid derivative of 750-A alkylamine C