Affinity chromatography of β-galactosidase on control-led-pore glass derivatives

✍ Scribed by George Baum

• Book ID: 104146029
• Publisher: Elsevier Science
• Year: 1975
• Tongue: English
• Weight: 499 KB
• Volume: 104
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)85493-5

✦ Synopsis

Severztl controlled-pore glslss (CPG) derivatives were examined as supports for the affinity chromatographic purification of the enzyme P-galactosidase. The competitive inhibitor p-aminophenyl$-D-thiogalactopyranoside was coupled to an azelaic acid and a malonic acid derivative of 750-A alkylamine CPG of 80-120 mesh and to an azelaic acid derivative of 550-A alkylamine CPG of 40-80 mesh. The latter derivative exhibited particularly good ,load capacity and separation efficiency; however, both arm lengths were effective. Hydrophobic interactions between the arm and the enzyme contribute to the separation.