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Controlled pore glass chromatography of protein-sodium dodecyl sulphate complexes

✍ Scribed by M.J. Frenkel; R.J. Blagrove

Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
512 KB
Volume
111
Category
Article
ISSN
1873-3778

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✦ Synopsis

The inclusion of urea has been found to eliminate adsorption of proteinsodium dodecyl sulphate (SDS) complexes to controlled pore glass. Using buffer containing 6 M urea, 0.5 "A, SDS and glass with pore diameter 12.3 nm, it is possible to determine protein molecular weights in the range 3500-12,000. Results with glass of larger pore diameter (25.5 nm) are similar to those reported in the absence of urea in the molecular-weight range 12,000-140,000. Controlled pore glass chromatography also permits the study of the relative importance of conformation free of charge effects for those proteins which deviate from the normal calibration curve for SDSpolyacrylamidc gels.

πŸ“œ SIMILAR VOLUMES

Protein-sodium dodecyl sulfate complexes
Protein-sodium dodecyl sulfate complexes: Determination of molecular weight, size and shape by controlled pore glass chromatography
✍ Robert C. Collins; Wolfgang Haller πŸ“‚ Article πŸ“… 1973 πŸ› Elsevier Science 🌐 English βš– 378 KB

The peak position vs log molecular weight curves of protein-SDS complexes chromatographed on controlled pore glass of narrow pore size distribution is linear over a molecular weight range of 17,000-385,000. A glass with a pore size of approximately 500 A allows the inclusion of all complexes in this