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Use of controlled-pore glass for adsorption chromatography of proteins

✍ Scribed by Takaharu Mizutani; Akira Mizutani

Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
339 KB
Volume
120
Category
Article
ISSN
1873-3778

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πŸ“œ SIMILAR VOLUMES

Controlled pore glass chromatography of
Controlled pore glass chromatography of protein-sodium dodecyl sulphate complexes
✍ M.J. Frenkel; R.J. Blagrove πŸ“‚ Article πŸ“… 1975 πŸ› Elsevier Science 🌐 English βš– 512 KB

The inclusion of urea has been found to eliminate adsorption of proteinsodium dodecyl sulphate (SDS) complexes to controlled pore glass. Using buffer containing 6 M urea, 0.5 "A, SDS and glass with pore diameter 12.3 nm, it is possible to determine protein molecular weights in the range 3500-12,000.

Adsorption of cationic biological materi
Adsorption of cationic biological materials on controlled pore glass
✍ Takaharu Mizutani; Akira Mizutani πŸ“‚ Article πŸ“… 1977 πŸ› Elsevier Science 🌐 English βš– 340 KB

Controlled pore glass (1 g, 97 m2) adsorbed approximately 5 wmol of cationic biological materials, such as lysine, histidine, arginine, hexosamines, and cytidine, in a distilled water medium. The amount of other amino acids and neutral carbohydrates adsorbed to controlled pore glass was O-O.8 WmoVg