Egg yolk lipoprotein promoted growth of a wide variety of mammalian cell lines, including plasma-cytomas and epithelial cell lines, in serum-free medium. The lipoprotein was active for cell growth when used with insulin, transferrin, ethanolamine and selenite. The most active lipoprotein fraction (Y
Chicken egg yolk-supplemented medium and the serum-free growth of normal mammalian cells
β Scribed by D. K. Fujii; D. Gospodarowicz
- Book ID
- 112797746
- Publisher
- Society for In Vitro Biology
- Year
- 1983
- Tongue
- English
- Weight
- 866 KB
- Volume
- 19
- Category
- Article
- ISSN
- 1475-2689
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Bovine milk contains growth promoting factors that stimulate DNA synthesis and cell division in confluent monolayers of quiescent Balb/c 3T3 cells. The growth factor activity was highest in colostrum obtained within 24 hours after birth of a calf. Samples of milk obtained 3 2 hours and 60 hours afte
A serum free medium was developed, that could be used for the large scale propagation of various cell lines in bioreactors. The medium is based on a 1:1 mixture oflscove's Modified Dulbecco's Medium and Ham's Medium F12, supplemented with transferrin, insulin and a BSAJoleic acid complex. Several my