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Serum-free growth medium for the cultivation of a wide spectrum of mammalian cells in stirred bioreactors

✍ Scribed by Volker Jäger; Jürgen Lehmann; Peter Friedl

Publisher
Springer
Year
1988
Tongue
English
Weight
680 KB
Volume
1
Category
Article
ISSN
0920-9069

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✦ Synopsis

A serum free medium was developed, that could be used for the large scale propagation of various cell lines in bioreactors. The medium is based on a 1:1 mixture oflscove's Modified Dulbecco's Medium and Ham's Medium F12, supplemented with transferrin, insulin and a BSAJoleic acid complex. Several myelomas, hybridomas derived from different myelomas and spleen cells, and other lymphoid and non-lymphoid cell lines were cultivated at growth rates comparable to those observed using serumsupplemented media. There was furthermore no reduction in the formation of products such as monoclonal antibodies or recombinant human interleukin-2.

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