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A novel serum-free medium for the cultivation of Vero cells on microcarriers

✍ Scribed by Chen Zhaolie; Xiao Chengzu; Liu Hong; Wu Benchuan; Jia Xihua

Publisher
Springer-Verlag
Year
1996
Tongue
English
Weight
635 KB
Volume
10
Category
Article
ISSN
0951-208X

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✦ Synopsis

A novel serum-free medium for the cultivation of Vero cells on microcarriers was developed,which composed of the 1: 1 mixture of Dubecco's Modified Eagle Medium : Nutrient Mixture F12, bovine serum albumin(BSA) or human serum albumin(HSA), epidermal growth factor(EGF), gelatin and Dbiotin. Both BSA and EGF were effective on cell growth, adhesion and spreading. Further addition of gelatin and biotin led to the enhanced cell adhesion and spreading without growth promoting activity. The serum-free medium was suitable for the cultivation of vero cells on several different microcarriers with cell density reached over 3x106cells/ml.

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