𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Influence of serum proteins on the kinetics of attachment of vero cells to cytodex microcarriers

✍ Scribed by Asok Mukhopadhyay; Satya N. Mukhopadhyay; Gursaran P. Talwar

Publisher
Wiley (John Wiley & Sons)
Year
2007
Tongue
English
Weight
546 KB
Volume
56
Category
Article
ISSN
0268-2575

No coin nor oath required. For personal study only.

✦ Synopsis

The attachment characteristics of the Vero cell line on microcarriers has been found to be influenced by the serum proteins. Significant quantities of serum proteins were found to be adsorbed on the carrier surface, which resulted in decreased exchange capacity and a lower attachment rate constant. The kinetics of attachment followed primarily first order with respect to the concentration of free cells. With positively charged microcarriers the attachment process exhibited two distinct phases, an initial phase of normal attachment followed by a second phase characterized by a lower rate of attachment. The attachment rate constant in the first phase (0.062 min-l) was reduced to 0.01 min-' in the second phase. Presumably, at a low concentration of free Vero cells, the attachment process is retarded by unadsorbed Bovine Serum Albumin (BSA) and other serum proteins present in the medium.

πŸ“œ SIMILAR VOLUMES

Effects of Serum on the Kinetics of CHO
Effects of Serum on the Kinetics of CHO Attachment to Ultraviolet–Ozone Modified Polystyrene Surfaces
✍ D.O.H. Teare; N. Emmison; C. Ton-That; R.H. Bradley πŸ“‚ Article πŸ“… 2001 πŸ› Elsevier Science 🌐 English βš– 70 KB

Attachment kinetics of Chinese hamster ovarian (CHO) cells were investigated on ultraviolet-ozone oxidized polystyrene (UVO-PS) dishes in the presence and absence of serum. The surface chemistry of UVO-PS has been extensively characterized. Although cells attached rapidly to the oxidized dishes with

The influence of transport on the kineti
The influence of transport on the kinetics of binding to surface receptors: application to cells and BIAcore
✍ Byron Goldstein; Daniel Coombs; Xiaoyi He; Angel R. Pineda; Carla Wofsy πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 819 KB

Accurate estimation of biomolecular reaction rates from binding data, when ligands in solution bind to receptors on the surfaces of cells or biosensors, requires an understanding of the contributions of both molecular transport and reaction. Efficient estimation of parameters requires relatively sim

Effects of serum and conditioned medium
Effects of serum and conditioned medium on protein degradation, migration of nonhistone proteins to the nucleus, and DNA synthesis in transformed cells
✍ Herman Polet; Jeffrey S. Swager πŸ“‚ Article πŸ“… 1987 πŸ› John Wiley and Sons 🌐 English βš– 811 KB

Stimulation of resting transformed cells (Chang liver cells), prelabeled with [3H] leucine, with fetal calf serum, caused increased nuclear translocation of [3H] nonhistone proteins ([3H] NHP) and DNA synthesis and a parallel inhibition of proteolysis of cellular proteins. [3H] NHP migration was ind