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The influence of transport on the kinetics of binding to surface receptors: application to cells and BIAcore

✍ Scribed by Byron Goldstein; Daniel Coombs; Xiaoyi He; Angel R. Pineda; Carla Wofsy

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
819 KB
Volume
12
Category
Article
ISSN
0952-3499

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✦ Synopsis

Accurate estimation of biomolecular reaction rates from binding data, when ligands in solution bind to receptors on the surfaces of cells or biosensors, requires an understanding of the contributions of both molecular transport and reaction. Efficient estimation of parameters requires relatively simple models. In this review, we give conditions under which various transport effects are negligible and identify simple binding models that incorporate the effects of transport, when transport cannot be neglected. We consider effects of diffusion of ligands to cell or biosensor surfaces, flow in a BIAcore biosensor, and distribution of receptors in a dextran layer above the sensor surface. We also give conditions under which soluble receptors can be expected to compete effectively with surface-bound receptors.

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