## Abstract For the cultivation of mammalian cells on microcarriers a minimum inoculum concentration is required to initiate cell attachment and subsequent cell growth. A critical cell number model has been proposed to elucidate the mechanism of the inoculum requirement. In this model it was hypoth
Selection of microcarrier diameter for the cultivation of mammalian cells on microcarriers
β Scribed by W.-S. Hu; D. I. C. Wang
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 1000 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0006-3592
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract A novel method of preparing collagen microcarriers was developed and used to entrap adherent cells for cell culturing. This new technique involved seeding of cells in micro gel beads comprised of collagen fibrils dispersed in alginate. The gel beads were washed with phosphate buffered s
## Abstract Quantitative image analysis has been applied to the monitoring of cultures of a mammalian cell line on microcarriers. Procedures have been developed to investigate microcarrier colonization and cluster formation and to determine the eventual modification of cell size during cultivation
The rates of cell attachment of the anchorage-dependent mammalian cell line Vero t o the gelatin-based macroporous microcarrier Cultispher-G were determined under various conditions. An optimal rate of attachment (0.98 x min-') occurred by an intermittent stirring regimen of 3 min stirring at 40 rpm
The maintenance of endothelial barrier function is important in the regulation of fluid and solute balance between the vascular space and the surrounding tissue. Since fluid flow across endothelial cells stimulates a wide variety of endothelial responses, the effect of shear stress on barrier functi