𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Application of quantitative image analysis to a mammalian cell line grown on microcarriers

✍ Scribed by Marie-Noëlle Pons; Anne Wagner; Harvé Vivier; Annie Marc

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
660 KB
Volume
40
Category
Article
ISSN
0006-3592

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Quantitative image analysis has been applied to the monitoring of cultures of a mammalian cell line on microcarriers. Procedures have been developed to investigate microcarrier colonization and cluster formation and to determine the eventual modification of cell size during cultivation using scanning electron microscopy (SEM) microphotography. The human kidney tumor cells (TCL 598) on which the procedures were tested underwent a slight size decrease during the development of the first cell layer on the microcarriers. The cluster size and the cell size remained constant during the culture stationary phase.

📜 SIMILAR VOLUMES

A mechanistic analysis of the inoculum r
A mechanistic analysis of the inoculum requirement for the cultivation of mammalian cells on microcarriers
✍ W.S. Hu; J. Meier; D.I.C. Wang 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 180 KB 👁 1 views

## Abstract For the cultivation of mammalian cells on microcarriers a minimum inoculum concentration is required to initiate cell attachment and subsequent cell growth. A critical cell number model has been proposed to elucidate the mechanism of the inoculum requirement. In this model it was hypoth

On-line monitoring of glucose in mammali
On-line monitoring of glucose in mammalian cell culture using a flow injection analysis (FIA) mediated biosensor
✍ Keith B. Male; P. Octavian Gartu; A. Amine Kamen; John H. T. Luong 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 200 KB 👁 2 views

A flow injection analysis (FIA) biosensor system has been developed for on-line determination of glucose during mammalian cell cultivation. The culture sample was peristaltically withdrawn from the bioreactor and after cell separation by a steam sterilizable ceramic microfilter, the filtrate was con

Development of an on-line monitoring sys
Development of an on-line monitoring system of human keratinocyte growth by image analysis and its application to bioreactor culture
✍ Masahiro Kino-oka; Jiri E. Prenosil 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 127 KB 👁 2 views

Human keratinocytes were cultured in serumfree medium for the purpose of on-line cell growth monitoring by image analysis. The validity of a process using a newly developed video microscopy system with image analysis for growth-rate monitoring in real time was verified by the measurement of the degr