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Chemiluminescence method for the determination of adenine after reaction with phenylglyoxal

✍ Scribed by Naotaka Kuroda; Kenichiro Nakashima; Shuzo Akiyama

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
351 KB
Volume
278
Category
Article
ISSN
0003-2670

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✦ Synopsis

A chemiluminescence (CL) method is described for the determination of adenine. Treatment of adenine with phenylglyoxal in acidic propan-2-01 gave products that produced CL in the presence of hydrogen peroxide, 2-mercaptoethanol and sodium hydroxide. The proposed method is highly sensitive and selective to adenine and adenosine. Adenine can be determined in the range 1.5 x 10-4-5.0 X lo-' M. The detection limit for adenine is 1.9 X lo-' M (19 pmol per assay).

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