Flow-injection chemiluminescence method for the determination of naphazoline hydrochloride and oxymetazoline hydrochloride

✍ Scribed by Nan-Nan Wang; Yan-Qing Shao; Yu-Hai Tang; He-Ping Yin; Xiao-Zhong Wu

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 235 KB
• Volume: 24
• Category: Article
• ISSN: 1522-7235
• DOI: 10.1002/bio.1092

✦ Synopsis

Abstract

A sensitive and simple flow‐injection chemiluminescence (FI‐CL) method, which was based on the CL intensity generated from the redoxreaction of potassium permanganate (KMnO~4~)–formaldehyde in vitriol (H~2~SO~4~) medium, has been developed, validated and applied for the determination of naphazoline hydrochloride and oxymetazoline hydrochloride. Besides oxidants and sensitizers, the effect of the concentration of H~2~SO~4~, KMnO~4~ and formaldehyde was investigated. Under the optimum conditions, the linear range was 1.0 × 10^−2^–7.0 mg/L for naphazoline hydrochloride and 5.0 × 10^−2^–10.0 mg/L for oxymetazoline hydrochloride. During seven repeated inter‐day and intra‐day precision tests of 0.1, 1.0 and 10.0 mg/L samples, the relative standard deviations all corresponded to reference values. The detection limit was 8.69 × 10^−3^ mg/L for naphazoline hydrochloride and 3.47 × 10^−2^ mg/L for oxymetazoline hydrochloride (signal‐to‐noise ratio ≤3). This method has been successfully implemented for the determination of naphazoline hydrochloride and oxymetazoline hydrochloride in pharmaceuticals. Copyright © 2009 John Wiley & Sons, Ltd.