A new sensitive flow-injection chemiluminescence method for the determination of H2-receptor antagonists

✍ Scribed by Yu-Hai Tang; Nan-Nan Wang; Xun-Yu Xiong; Feng-Mei Xiong; Si-Juan Sun

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 221 KB
• Volume: 22
• Category: Article
• ISSN: 1522-7235
• DOI: 10.1002/bio.969

✦ Synopsis

Abstract

Based on the chemiluminescence (CL) intensity generated from the potassium ferricyanide [K~3~Fe(CN)~6~]–rhodamine 6G system in sodium hydroxide (NaOH) medium, a new sensitive flow‐injection chemiluminescence (FI–CL) method has been developed, validated and applied for the determination of three kinds of H~2~‐receptor antagonists: cimetidine (CIMT), ranitidine (RANT) hydrochloride and famotidine (FAMT). Under the optimum conditions, the linear range for the determination was 1.0 × 10^−9^–7.0 × 10^−5^ g/ml for CIMT, 1.0 × 10^−9^–5.0 × 10^−5^ g/mL for RANT hydrochloride and 5.0 × 10^−9^–7.0 × 10^−5^ g/mL for FAMT. During 11 repeated measurements of 1.0 × 10^−6^ g/mL sample solutions, the relative standard deviations (RSDs) were all <5%. The detection limit was 8.56 × 10^−10^ g/mL for CIMT, 8.69 × 10^−10^ g/mL for RANT hydrochloride and 2.35 × 10^−9^ g/mL for FAMT (S:N = 3). This method has been successfully implemented for the analysis of H~2~‐receptor antagonists in pharmaceuticals. Copyright © 2007 John Wiley & Sons, Ltd.