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A novel chemiluminescence method for the determination of orciprenaline based on ferricyanide-rhodamine 6G

✍ Scribed by Yi Lv; Rui Zhao; Zhenli Zhu; Xianshun Xu; Xinrong Zhang

Publisher: John Wiley and Sons
Year: 2005
Tongue: English
Weight: 114 KB
Volume: 20
Category: Article
ISSN: 1522-7235
DOI: 10.1002/bio.860

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✦ Synopsis

Abstract

A novel flow injection chemiluminescence method for the determination of orciprenaline was developed. The method is based on the chemiluminescence (CL) reaction of orciprenaline with potassium ferricyanide in sodium hydroxide medium, sensitized by the fluorescent dye rhodamine 6G. The proposed procedure allows quantitation of orciprenaline in the concentration range 0.01–1.2 µg/mL, with a detection limit of 7.2 × 10^−3^ µg/mL. The relative standard deviation (RSD) is 2.7% for 0.1 µg/mL orciprenaline (n = 9). The sampling frequency was calculated at ∼120/h. The method was successfully applied to the determination of orciprenaline in pharmaceutical preparations. A brief discussion on the possible CL reaction mechanism is presented. Copyright © 2005 John Wiley & Sons, Ltd.

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