Chemiluminescence enzyme immunoassay of cortisol using peroxidase as label

✍ Scribed by Hidetoshi Arakawa; Masako Maeda; Akio Tsuji

• Book ID: 102984346
• Publisher: Elsevier Science
• Year: 1979
• Tongue: English
• Weight: 544 KB
• Volume: 97
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(79)90352-x

✦ Synopsis

A new and highly sensitive enzyme immunoassay of cortisol was established using horseradish peroxidase as the label. Separation of free and bound cortisol was effected by insolubilized anti-cortisol antibody which was prepared by coupling the purified immunoglobulin G of antiserum with Sepharose 4B. The enzyme activity was measured by the chemiluminescence reaction using luminol and hydrogen peroxide as substrate. The faint chemiluminescence was measured by a photon counter. Comparison of assay results obtained by radioimmunoassay and this enzyme immunoassay showed excellent agreement of results in all cases (r = 0.913). The detection limit of cortisol was about 1Opgper assay tube. This enzyme immunoassay,is applicable to the routine determination of plasma cortisol.