Chemiluminescence enzyme immunoassay of
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Hidetoshi Arakawa; Masako Maeda; Akio Tsuji
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Article
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1979
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Elsevier Science
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English
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A new and highly sensitive enzyme immunoassay of cortisol was established using horseradish peroxidase as the label. Separation of free and bound cortisol was effected by insolubilized anti-cortisol antibody which was prepared by coupling the purified immunoglobulin G of antiserum with Sepharose 4B.