Chemiluminescence assay of alkaline phosphatase using cortisol-21 -phosphate as substrate and its application to enzyme immunoassays

✍ Scribed by Amane Kokado; Akio Tsuji; Masako Maeda

• Publisher: Elsevier Science
• Year: 1997
• Tongue: English
• Weight: 401 KB
• Volume: 337
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/s0003-2670(96)00400-x

✦ Synopsis

A method is described for the highly sensitive chemiluminescence (CL) assay for alkaline phosphatase (ALP) using cortisol-21-phosphate (cortisol-21-P) as substrate and lucigenin as CL reagent; it is applied to an enzyme immunoassay (EIA) for human chorionic gonadotropin (hCG), human growth hormone (hGH), n-fetoprotein (AFP) and estradiol (E2). The detection limit for ALP was 8.75 x 10-20 mol/assay, and the average coefficient of variation (n=l 1) over the calibration range (3.55x 10-~'5-7.1 x 10-'Omol I-') was 6.4%. In EIA for hCG. hGH, AFP and E2, the detection limits were 0.008 mIU ml '.

10. 40 and 0.25 pgml ~', respectively.