✦ LIBER ✦

Characterization of the secretion efficiency of a plant signal peptide in Bacillus subtilis

✍ Scribed by Ribbe, Joachim ;Nagarajan, Vasantha

Publisher: Springer
Year: 1992
Tongue: English
Weight: 847 KB
Volume: 235
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00279378

No coin nor oath required. For personal study only.

✦ Synopsis

The ability of the Bacillus subtilis secretion machinery to interact with a heterologous signal peptide was studied using a plant (wheat alpha-amylase) signal peptide. The plant signal peptide was capable of mediating secretion of Escherichia coli alkaline phosphatase and B. amyloliquefaciens levansucrase from B. subtilis. This secretion was dependent on the plant signal peptide, as deletion of five amino acids from the hydrophobic core resulted in a block of secretion. Attempts to improve the efficiency of the plant signal peptide in B. subtilis were made by increasing the length of the hydrophobic core from 10 to 16 residues by insertion of 2, 4, 5 or 6 amino acids. None of the alterations improved the secretion efficiency relative to the wild-type plant signal peptide.

📜 SIMILAR VOLUMES

Expression and Secretion of an Acid-Stab

Expression and Secretion of an Acid-Stableα-Amylase Gene in Bacillus Subtilis bySacBPromoter and Signal Peptide

✍ Cai Heng; Zhongjun Chen; Lianxiang Du; Fuping Lu 📂 Article 📅 2005 🏛 Springer Netherlands 🌐 English ⚖ 295 KB

The roles of signal peptide and mature p

The roles of signal peptide and mature protein in RNase (barnase) export from Bacillus subtilis

✍ Chen, Mario ;Nagarajan, Vasantha 📂 Article 📅 1993 🏛 Springer 🌐 English ⚖ 877 KB

Barnase, an extracellular RNAse from Bacillus amyloliquefaciens is secreted post-translationally from B. subtilis. The rate of secretion of barnase from B. subtilis was improved by replacement of the barnase signal peptide with a heterologous signal peptide. However, the barnase signal peptide expor

The phosphorelay signal transduction pat

The phosphorelay signal transduction pathway in the initiation of Bacillus subtilis sporulation

✍ James A. Hoch 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 624 KB

The formation of spores in Bacillus subtrhs is a developmental process under genetic control. The decision to either divide or sporulate is regulated by the state of phosphorylation of the SpoOA transcription factor. Phosphorylated SpoOA (SpoOA -P) is both a repressor and an activator of transcripti

Synthesis and secretion of a heat-stable

Synthesis and secretion of a heat-stable carboxymethylcellulase from Clostridium thermocellum in Bacillus subtilis and Bacillus stearothermophilus

✍ Soutschek-Bauer, Erwin ;Staudenbauer, Walter L. 📂 Article 📅 1987 🏛 Springer 🌐 English ⚖ 486 KB

The cellulase gene celA of Clostridium thermocellum coding for the thermostable endoglucanase A was transferred from Escherichia coli to Bacillus subtilis 168 and B. stearothermophilus CU21 using plasmids derived from the Bacillus vector pUB110. When the structural part of the gene was joined to a p

Modulation of the spc operon affects gro

Modulation of the spc operon affects growth and protein secretion in Bacillus subtilis

✍ Reinhard Breitling; Bernhard Schlott; Detlev Behnke 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 768 KB

A proximal segment of B.subtilis secY gene was placed under the control of the inducible .spuc. promoter/Lac repressor system. This fusion was integrated into the chromosomal spc operon of B. subtilis via CAMPBELL-like reciprocal recombination. The growth of the resulting strain was strongly IPTG de

Advances in the use of Bacillus subtilis

Advances in the use of Bacillus subtilis for the expression and secretion of heterologous proteins

✍ Sui-Lam Wong 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 641 KB