Recombinant baculoviruses are widely used to express heterologous proteins. They are particularly useful for the high-level expression of eukaryotic proteins with fastidious co-or post-translational processing requirements. The recent publication of the Autographa californica nuclear polyhedrosis vi
Advances in the use of Bacillus subtilis for the expression and secretion of heterologous proteins
β Scribed by Sui-Lam Wong
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 641 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0958-1669
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A proximal segment of B.subtilis secY gene was placed under the control of the inducible .spuc. promoter/Lac repressor system. This fusion was integrated into the chromosomal spc operon of B. subtilis via CAMPBELL-like reciprocal recombination. The growth of the resulting strain was strongly IPTG de
The previously cloned and sequenced streptokinase gene (skc) from Streptococcus equisimilis H46A was inserted into plasmid vectors capable of replication in Bacillus subtilis. The skc gene was expressed by use of its own transcription and translation signals which appeared to meet the stringent requ
## Abstract A novel metabolic engineering technique involving the redirection ofcellular carbon fluxes was employed to reduce acetate production in an __Escherichia coli__ culture. Metabolic engineering was achieved by cloning __E. coli__ the gene for the __Bacillus subtilis__ acetolactate synthase