✦ LIBER ✦

Modulation of the spc operon affects growth and protein secretion in Bacillus subtilis

✍ Scribed by Reinhard Breitling; Bernhard Schlott; Detlev Behnke

Publisher: John Wiley and Sons
Year: 1994
Tongue: English
Weight: 768 KB
Volume: 34
Category: Article
ISSN: 0233-111X
DOI: 10.1002/jobm.3620340303

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✦ Synopsis

A proximal segment of B.subtilis secY gene was placed under the control of the inducible .spuc. promoter/Lac repressor system. This fusion was integrated into the chromosomal spc operon of B. subtilis via CAMPBELL-like reciprocal recombination. The growth of the resulting strain was strongly IPTG dependent. With staphylokinase and a-amylase as reporter proteins it was found, that the protein secretion capacity of this strain was correlated to the conditions of repression or induction of the chromosomal spac promoter.

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