✦ LIBER ✦

The roles of signal peptide and mature protein in RNase (barnase) export from Bacillus subtilis

✍ Scribed by Chen, Mario ;Nagarajan, Vasantha

Publisher: Springer
Year: 1993
Tongue: English
Weight: 877 KB
Volume: 239
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00276939

No coin nor oath required. For personal study only.

✦ Synopsis

Barnase, an extracellular RNAse from Bacillus amyloliquefaciens is secreted post-translationally from B. subtilis. The rate of secretion of barnase from B. subtilis was improved by replacement of the barnase signal peptide with a heterologous signal peptide. However, the barnase signal peptide exported Escherichia coli alkaline phosphatase faster than mature barnase. Heat shock of B. subtilis cells did not significantly alter the export of barnase using the barnase signal peptide. The slow rate of export of barnase from B. subtilis is due to both the signal peptide and the mature protein sequence rather than either alone.

📜 SIMILAR VOLUMES

Modification of length, hydrophobic prop

Modification of length, hydrophobic properties and electric charge of Bacillus subtilis α-amylase signal peptide and their different effects on the production of secretory proteins in B. subtilis and Escherichia coli cells

✍ Nakamura, Kouji ;Fujita, Yasuhito ;Itoh, Yoshiki ;Yamane, Kunio 📂 Article 📅 1989 🏛 Springer 🌐 English ⚖ 977 KB