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Characterization of sister chromatid exchange induction by hydrogen peroxide

✍ Scribed by Speit, G. ;Vogel, W. ;Wolf, M.

Publisher
Wiley (John Wiley & Sons)
Year
1982
Weight
465 KB
Volume
4
Category
Article
ISSN
0192-2521

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✦ Synopsis

Hydrogen peroxide (H,O,) induces SCEs in V-79 Chinese hamster cells. The incorporation of BrdUrd sensitizes the cells to the action of H,O,, but damage leading to the formation of SCEs can also be induced in DNA not substitued with BrdUrd. Catalase leads to a complete reduction of the SCE-producing as well as the toxic effect. The amino acid L-cysteine does not reduce the H20,-induced SCEfrequencies. The results yield information about the relevance of SCE investigations in vitro for the determination of DNA-damaging substances and about the effects of antimutagenic SH-compounds.

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