Characterization of rapid and high-affinity uptake of L-serine in neurons and astrocytes in primary culture
β Scribed by Toshifumi Yamamoto; Itone Nishizaki; Shigeki Furuya; Yoshio Hirabayashi; Kenzo Takahashi; Shigeru Okuyama; Hideko Yamamoto
- Book ID
- 117105618
- Publisher
- Elsevier Science
- Year
- 2003
- Tongue
- English
- Weight
- 157 KB
- Volume
- 548
- Category
- Article
- ISSN
- 0014-5793
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Uptake and oxidative metabolism of [I4C]malate as well as its incorporation into aspartate, glutamate, glutamine, and GABA were studied in cultured cerebral cortical neurons (GABAergic), cerebellar granule neurons (glutamatergic), and cerebral cortical astrocytes. All cell types exhibited high affin
The uptake, release, and metabolism of alanine were studied in primary cultures of cerebral cortical neurons or astrocytes and cerebellar granule neurons. All three cell types exhibited a saturable, sodiumdependent uptake of alanine with K, values (pM) of 256 f 30,463 f 39, and 292 f 39, respectivel