Metabolic fate of [8-14C]adenosine was studied in primary cultures of either astrocytes or neurons from the mouse brain. In astrocytes the main metabolic route was the formation of nucleotides. Thus, synthesis of adenosine triphosphate (ATP) amounted to about 0.2 nmol X min-1 X mg-1 protein. The dea
Similarities of adenosine uptake systems in astrocytes and neurons in primary cultures
β Scribed by Alexander S. Bender; Leif Hertz
- Publisher
- Springer
- Year
- 1986
- Tongue
- English
- Weight
- 944 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0364-3190
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
The question whether the benzodiazepine receptor site in astrocytes or in neurons might be identical to the adenosine uptake site was studied by determining pharmacological profiles, inhibition types, and the effects of benzodiazepine antagonists in primary cultures of either astrocytes or neurons.
Uptake and oxidative metabolism of [I4C]malate as well as its incorporation into aspartate, glutamate, glutamine, and GABA were studied in cultured cerebral cortical neurons (GABAergic), cerebellar granule neurons (glutamatergic), and cerebral cortical astrocytes. All cell types exhibited high affin
The uptake, release, and metabolism of alanine were studied in primary cultures of cerebral cortical neurons or astrocytes and cerebellar granule neurons. All three cell types exhibited a saturable, sodiumdependent uptake of alanine with K, values (pM) of 256 f 30,463 f 39, and 292 f 39, respectivel
The regulation of intracellular cyclic AMP (CAMP) formation by adenosine (Ado) and its analogues has been examined in primary cultures of rat-brain astrocytes and neurons. In the presence of the phosphodiesterase inhibitor, Ro 20-1724, basal levels of cAMP ranged from 40-120 pmoVmg protein in both c