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Characterization of DNA polymerase δ from a higher plant, rice (Oryza sativa L.)

✍ Scribed by Yukinobu Uchiyama; Masami Hatanaka; Seisuke Kimura; Toyotaka Ishibashi; Tadamasa Ueda; Yoshikiyo Sakakibara; Takashi Matsumoto; Tomoyuki Furukawa; Junji Hashimoto; Kengo Sakaguchi


Book ID
117521005
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
323 KB
Volume
295
Category
Article
ISSN
0378-1119

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Lipase from defatted rice bran has been isolated by a new procedure which yields higher enzyme activity and quantity of enzyme. The procedure involves selective ammonium sulphate precipitation followed by ion exchange chromatography using DEAE-Sepharose CL-6B gel and column chromatography. The purif