Characterization of bromine-77-labeled proteins prepared using myeloperoxidase
β Scribed by Karen D. McElvany; John W. Barnes; Michael J. Welch
- Book ID
- 103114235
- Publisher
- Elsevier Science
- Year
- 1980
- Weight
- 770 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0020-708X
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## Abstract A tumorβassociated monoclonal antibody, named 7E8 and raised against human placental alkaline phosphatase (hPLAP), is labelled with bromineβ77 by means of chloramineβT. The paper describes optimum radiobromination conditions resulting in 34% radiochemical yield of labelled antibody with
Three 7-aminocoumarin derivatives, 7-carboxymethylamino-4-trifluoromethyl-2H-1-benzopyran-2-one [2], 7-carboxymethylamino-4-methyl-2H-1-benzopyrank2-one [4], and 7-dimethylamino-4-formyl-2H-1-benzopyran-2-one [6], were prepared. The reagents can be attached to primary amino groups of proteins. The l