CHARACTERIZATION OF FISH MUSCLE PROTEASES USING RADIO-LABELED PROTEIN SUBSTRATES

Fluorometric determination of bacterial

Fluorometric determination of bacterial protease activity using fluorescein isothiocyanate-labeled proteins as substrates

✍ Karen A. Homer; David Beighton 📂 Article 📅 1990 🏛 Elsevier Science 🌐 English ⚖ 549 KB

Intact fluorescein isothiocyanate-labeled proteins have relatively low background fluorescence at excitation and emission wavelengths of 495 and 525 nm, respectively. Degradation of these substrates leads to exposure of covalently linked fluorescein isothiocyanate molecules and to a concomitant incr