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Preparation of bromine-77 labelled monoclonal anti-hPLAP antibody using chloramine-T

✍ Scribed by Fabienne Lambert; Guido Slegers; Patrick Goethals


Publisher
John Wiley and Sons
Year
1991
Tongue
French
Weight
258 KB
Volume
29
Category
Article
ISSN
0022-2135

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✦ Synopsis


Abstract

A tumor‐associated monoclonal antibody, named 7E8 and raised against human placental alkaline phosphatase (hPLAP), is labelled with bromine‐77 by means of chloramine‐T. The paper describes optimum radiobromination conditions resulting in 34% radiochemical yield of labelled antibody with more than 90% immunoreactivity.