Preparation and Characterization of Three Fluorescent Labels for Proteins, Suitable for Structural Studies

✍ Scribed by Y. Forster; E. Haas

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 490 KB
• Volume: 209
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1993.1076

✦ Synopsis

Three 7-aminocoumarin derivatives, 7-carboxymethylamino-4-trifluoromethyl-2H-1-benzopyran-2-one [2], 7-carboxymethylamino-4-methyl-2H-1-benzopyrank2-one [4], and 7-dimethylamino-4-formyl-2H-1-benzopyran-2-one [6], were prepared. The reagents can be attached to primary amino groups of proteins. The labeled derivatives are stable and are characterized by a strong absorption band in the near uv ( (300-400 \mathrm{~nm}) ) and an emission band in the blue region ( (440-510 \mathrm{~nm}) ) with a large Stokes' shift. The spectral characteristics of these probes make them useful in applications of (\mathrm{dy}-) namic nonradiative excitation energy transfer measurements for conformational studies and analytical procedures based on simultaneous detection of labeled components in mixtures. c 1993 Academic Press, Inc.