Characterization of a fourth streptococcal pyrogenic exotoxin (SPE D)
โ Scribed by Ray A. McMillian; Timothy A. Bloomster; Abdul M. Saeed; Kristina L. Henderson; Nancy E. Zinn; Roy Abernathy; Dennis W. Watson; Richard N. Greenberg
- Book ID
- 109313946
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 840 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0378-1097
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A physical map of group A streptococcal bacteriophage T12 was constructed with restriction endonucleases Sal/I, PstI, and EcoRI. The map is circularly permuted with a total length of 36.0 kb. Sub-molar quantities of certain restriction fragments, some of very precise MW and some of heterogeneous MW,
The genes encoding streptococcal pyrogenic exotoxin type A (SPE A) and staphylococcal toxic shock syndrome toxin-1 (TSST-1) were stably cloned and expressed in Bacillus subtilis. In the non-pathogenic Bacillus background, the recombinant speA clone expressed 32-fold more SPE A than the native strept