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A physical map of the group A streptococcal pyrogenic exotoxin bacteriophage T12 genome

✍ Scribed by Johnson, Lane P. ;Schlievert, Patrick M.


Publisher
Springer
Year
1983
Tongue
English
Weight
942 KB
Volume
189
Category
Article
ISSN
0026-8925

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✦ Synopsis


A physical map of group A streptococcal bacteriophage T12 was constructed with restriction endonucleases Sal/I, PstI, and EcoRI. The map is circularly permuted with a total length of 36.0 kb. Sub-molar quantities of certain restriction fragments, some of very precise MW and some of heterogeneous MW, were observed. This observation, together with mapping data, suggests that DNA packaging is initiated at a precise site on a concatemeric precursor and proceeds for a limited number of rounds.


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