The structural gene for group A streptococcal pyrogenic exotoxin (SPE) type A was cloned into E. coli. DNA fragments used for cloning the toxin gene were isolated from bacteriophage T12. Toxin, present in cell lysates of E. coli clones, immunoprecipitated with antisera raised against purified SPE ty
A physical map of the group A streptococcal pyrogenic exotoxin bacteriophage T12 genome
β Scribed by Johnson, Lane P. ;Schlievert, Patrick M.
- Publisher
- Springer
- Year
- 1983
- Tongue
- English
- Weight
- 942 KB
- Volume
- 189
- Category
- Article
- ISSN
- 0026-8925
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β¦ Synopsis
A physical map of group A streptococcal bacteriophage T12 was constructed with restriction endonucleases Sal/I, PstI, and EcoRI. The map is circularly permuted with a total length of 36.0 kb. Sub-molar quantities of certain restriction fragments, some of very precise MW and some of heterogeneous MW, were observed. This observation, together with mapping data, suggests that DNA packaging is initiated at a precise site on a concatemeric precursor and proceeds for a limited number of rounds.
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