✦ LIBER ✦

Physical maps of the streptococcal bacteriophage A25 and C1 genomes

✍ Scribed by Mary E. Pomrenke; Prof. Dr. Joseph J. Ferretti

Publisher: John Wiley and Sons
Year: 1989
Tongue: English
Weight: 231 KB
Volume: 29
Category: Article
ISSN: 0233-111X
DOI: 10.1002/jobm.3620290621

No coin nor oath required. For personal study only.

✦ Synopsis

A physical map of the group A streptococcal bacteriophage A25 genome was constructed using data obtained from restriction enzyme digestion analysis of purified DNA. Phage A25 was 34.6 kb in size and was digested with several enzymes which generated the following number of fragments: AvuI (3), EcoRI (2), Hind11 (4), Hue11 (lo), Hind1 ( 22), HpaI (Il), and PvuII (8). Restriction enzymes BamH1, PstI, and Sun did not cleave phage A25 DNA. A physical map of the group C streptococcal phage C1 genome was also constructed. Phage C1 was 16.9 kb in size and was cleaved by the following enzymes to give the indicated number of fragments; EcoRI (8), Hue11 (4), Hue111 (lo), HindIII (5), HpaI (8), PvuII (3), and Sun (2). Restriction enzymes AvuI, AvuII, BumH1, EcoRV, HpaII, and PsrI did not cleave phage C1 DNA.

📜 SIMILAR VOLUMES

A physical map of the group A streptococ

A physical map of the group A streptococcal pyrogenic exotoxin bacteriophage T12 genome

✍ Johnson, Lane P. ;Schlievert, Patrick M. 📂 Article 📅 1983 🏛 Springer 🌐 English ⚖ 942 KB

A physical map of group A streptococcal bacteriophage T12 was constructed with restriction endonucleases Sal/I, PstI, and EcoRI. The map is circularly permuted with a total length of 36.0 kb. Sub-molar quantities of certain restriction fragments, some of very precise MW and some of heterogeneous MW,

A physical map of the permuted genome of

A physical map of the permuted genome of bacteriophage T1

✍ Ramsay, Neil ;Ritchie, Donald A. 📂 Article 📅 1980 🏛 Springer 🌐 English ⚖ 647 KB

A restriction map has been constructed for the DNA of coliphage T1 which locates the cleavage sites of the restriction endonucleases, BglI (6 cuts), BglII (16 cuts), EcoRI (2 cuts), HindIII (2 cuts) and PstI (2 cuts). Digestions with BglI and BglII reveal fragments which are present in sub-molar qua

Multiple physical differences in the gen

Multiple physical differences in the genome structure of functionally related bacteriophages P1 and P7

✍ Iida, Shigeru ;Arber, Werner 📂 Article 📅 1979 🏛 Springer 🌐 English ⚖ 968 KB

Comparative restriction cleavage analysis of the genomes of bacteriophage P7, of several recombinant phages between P7 and P1, and of bacteriophage P1 allowed to draw PstI, Bg/II, BamHI and HindIII cleavage maps of all genomes studied. The data obtained complement Yun and Vapnek's (1977) conclusions

The chloroplast genome of the gymnosperm

The chloroplast genome of the gymnospermPinus contorta: a physical map and a complete collection of overlapping clones

✍ Jonas Lidholm; Petter Gustafsson 📂 Article 📅 1991 🏛 Springer-Verlag 🌐 English ⚖ 731 KB

Overlapping restriction fragments of chloroplast DNA from the conifer Pinus contorta were cloned. Out of a total of 49 clones, 33 comprise the minimum set required to represent the entire genome. Using the purified inserts of these clones as probes in filter hybridizations, all sites for the three r

A physical map of bacteriophage T4 inclu

A physical map of bacteriophage T4 including the positions of strong promoters and terminators recognized in vitro

✍ Gram, Hermann ;Liebig, Hans-Dieter ;Hack, Alfons ;Niggemann, Elisabeth ;Rüger, W 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 971 KB

We present a linearized physical map of the genome of bacteriophage T4. This map contains the cleavage sites for restriction enzymes SmaI, KpnI, SalI, BglII, XhoI, XbaI, ClaI , HaeII, EcoRI, and EcoRV . It also contains about 200 TaqI sites. The promoter sites recognized in vitro and a number of rho

Correlation of physical maps and some ge

Correlation of physical maps and some genetic functions in the genomes of the κ-ϑ phage family of Bacillus licheniformis

✍ Doskočil, Jiří ;Štorchová, Helena ;Štokrová, Jitka ;Forstová, Jitka ;Meyer, Jürg 📂 Article 📅 1988 🏛 Springer 🌐 English ⚖ 665 KB

Natural recombinant genomes between several, phenotypically distinct forms of phages kappa and theta were isolated and characterized by DNA restriction fragment mapping and electron microscopic heteroduplex analysis. The phenotypes of the recombinants were correlated with the physical maps of the ge