𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Cloning and expression of streptococcal pyrogenic exotoxin A and staphylococcal toxic shock syndrome toxin-1 in Bacillus subtilis

✍ Scribed by Kreiswirth, B. N. ;Handley, J. P. ;Schlievert, P. M. ;Novick, R. P.

Book ID
104725088
Publisher
Springer
Year
1987
Tongue
English
Weight
459 KB
Volume
208
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.

✦ Synopsis

The genes encoding streptococcal pyrogenic exotoxin type A (SPE A) and staphylococcal toxic shock syndrome toxin-1 (TSST-1) were stably cloned and expressed in Bacillus subtilis. In the non-pathogenic Bacillus background, the recombinant speA clone expressed 32-fold more SPE A than the native streptococcus, and similarly, the recombinant plasmid harboring tst expressed 4-fold more TSST-1 in Bacillus than in the native Staphylococcus aureus. The Bacillus-derived products were secreted into the culture fluid, were resistant to proteolytic degradation and their biological activities mimicked native preparations.

📜 SIMILAR VOLUMES

Specific immuno capturing of the Staphyl
Specific immuno capturing of the Staphylococcal superantigen toxic-shock syndrome toxin-1 in plasma
✍ Hendrik Adams; Walter Brummelhuis; Bram Maassen; Nathalie van Egmond; Mohamed El 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 406 KB

## Abstract Toxic‐shock syndrome is primarily caused by the Toxic‐shock syndrome toxin 1 (TSST‐1), which is secreted by the Gram‐positive bacterium __Staphylococcus aureus__. The toxin belongs to a family of superantigens (SAgs) which exhibit several shared biological properties, including the indu