Molecular basis of group A streptococcal pyrogenic exotoxin B
โ Scribed by Hideaki Nagamune; Kazuto Ohkura; Hisashi Ohkuni
- Book ID
- 106287126
- Publisher
- Springer
- Year
- 2005
- Tongue
- English
- Weight
- 482 KB
- Volume
- 11
- Category
- Article
- ISSN
- 1341-321X
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๐ SIMILAR VOLUMES
A physical map of group A streptococcal bacteriophage T12 was constructed with restriction endonucleases Sal/I, PstI, and EcoRI. The map is circularly permuted with a total length of 36.0 kb. Sub-molar quantities of certain restriction fragments, some of very precise MW and some of heterogeneous MW,
The structural gene for group A streptococcal pyrogenic exotoxin (SPE) type A was cloned into E. coli. DNA fragments used for cloning the toxin gene were isolated from bacteriophage T12. Toxin, present in cell lysates of E. coli clones, immunoprecipitated with antisera raised against purified SPE ty